Ni Sepharose™ 6Fast Flow
SIGMA/GE17-5318-02 - Cytiva 17-5318-02, pack of 100 mL
Synonym: Ni-Sepharose Fast Flow; Nickel Affinity Resin; Nickel Column
Product Type: Chemical
| average diameter | 90 μm |
| capacity | ~40 mg binding capacity (histidine-tagged protein / mL resin) |
| cleaning in place | 2-14(Ni2+-stripped medium.) |
| crosslinking | cross-linked |
| form | resin () |
| manufacturer/tradename | Cytiva 17-5318-02 |
| matrix | 6% cross-linked agarose |
| matrix active group | Ni2+ ions |
| packaging | pack of 100 mL |
| parameter | 150 cm/hr flow rate |
| particle size | 45-165 μm |
| separation technique | affinity |
| shipped in | ambient |
| storage condition | (20% Ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12(Ni2+-stripped medium.) |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Designed for scaling up purification of histidine-tagged proteins • High protein binding capacity and minimal leakage of Ni2? ions • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support. • Available in convenient prepacked HisPrep™ and HisTrap™ column formats. |
| General description: | Ni Sepharose™ 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose™ Fast Flow platform. Ni Sepharose™ 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification. Ni Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.. Ni Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As A member of the BioProcess media range, Ni Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support. |
| Legal Information: | HisPrep is a trademark of Cytiva |
| Legal Information: | HisTrap is a trademark of Cytiva |
| Legal Information: | IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Storage Temp.: 4°C to 30°C |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41106500 |