HisTrap™ Fast Flow
SIGMA/GE17-5319-01 - Cytiva 17-5319-01, pack of 5 × 1 mL
Synonym: HIS chromatography column; HIS-tagged purification columns; IMAC column; Ni2+ Sepharose FF; immobilized metal ion chromatography column; prepacked column
Product Type: Chemical
HisTrap FF
| average diameter | 90 μm |
| bed I.D. × L | 7 mm × 25 mm |
| bed volume | 1 mL |
| capacity | ~40 mg binding capacity (histidine-tagged protein / mL) |
| cleaning | 2-14(Ni2+-stripped medium.) |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 17-5319-01 |
| matrix | 6% cross-linked agarose |
| matrix active group | Ni2+ ions |
| packaging | pack of 5 × 1 mL |
| parameter | 1 mL/min flow rate (recommended) |
| 4 mL/min max. flow rate | |
| 5 bar max. pressure | |
| particle size | 45-165 μm |
| separation technique | affinity |
| shipped in | ambient |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12(Ni2+-stripped medium.) |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • High binding capacity, approx. 40 mg/mL medium. • Negligible leakage of Ni2+. • Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins. • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives. |
| General description: | HisTrap™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications. The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols. |
| Legal Information: | HisTrap is a trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-30°C |
| UNSPSC | 23151816 |