IPG-Buffer, pH 3-10 NL
SIGMA/GE17-6000-88 - Cytiva 17-6000-88
Synonym: 2D electrophoresis buffer; Immobiline buffer; isoelectric focusing buffer; reagents
Product Type: Chemical
IPG Buffer pH 3–10 NL
| color | Colorless to light yellow |
| feature | wetted part: no |
| form | liquid |
| manufacturer/tradename | Cytiva 17-6000-88 |
| packaging | pkg of 1 mL |
| pH range | 3-10 |
| solubility | cold water: soluble (Soluble in cold water and hot water) |
| sterility | non-sterile |
| storage temp. | 2-8°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Each IPG Buffer type produces more uniform conductivity along the Immobiline® DryStrip during focusing, resulting in wider latitude in run times. IPG Buffers also eliminate high background staining. IPG buffers are supplied in 1-ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip. |
| Features and Benefits: | • For use with Immobiline® DryStrip gels to improve Protein solubility. • Using Immobiline® DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting Low abundance Proteins. • Improved IPG buffer pH 3-10 and pH 3-10 NL offer improved resolution, sensitivity, and increased spot intensity as well as additional Protein spots in high molecular weight and basic pH areas. • Eliminates background staining. • Ensures optimal conditions for IEF separation. |
| General description: | IPG Buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline® DryStrip gels. |
| Legal Information: | Immobiline is a registered trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41105323 |