HiTrap® IEX Selection Kit
SIGMA/GE17-6002-33 - Cytiva 17-6002-33
Synonym: IEX Selection Kit
Product Type: Chemical
| average diameter | 90 μm |
| bed size | 7 mm × 25 mm |
| bed volume | 1 mL |
| cleaning | 2-14 |
| column I.D. | 7 mm |
| description | Ion Exchanger Type (value) |
| ligand | quaternary amine |
| manufacturer/tradename | Cytiva 17-6002-33 |
| matrix | 6% cross-linked agarose |
| packaging | pkg of 7 ea |
| parameter | 42 psi |
| particle size | 45-165 μm |
| suitability | suitable for bioprocess medium |
| working range | 2-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1 mL HiTrap® columns. • Offers a fast, easy and convenient way screen for the optimal media for a given application . • Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up. • Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design. |
| General description: | HiTrap® IEX Selection Kit consists of seven HiTrap® columns, prepacked with different Sepharose™ Fast Flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work. Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)) |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| UNSPSC | 23151816 |