Microspin™ G-50 Columns
SIGMA/GE27-5330-02 - Cytiva 27-5330-02, pack of 250 columns
Synonym: Amersham spin column; DNA purification columns; Microspin columns; PCR cleanup columns; size exclusion columns; spin column
Product Type: Chemical
| manufacturer/tradename | Cytiva 27-5330-02 |
| material | resin (Sephadex G-50 DNA Grade) |
| packaging | pack of 250 columns |
| particle size | 50-150 μm (dry) |
| pore size | 30 kDa MWCO |
| sterility | non-sterile |
| working volume | 12—50 μL |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography. • Columns are prepacked with Sephadex® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide. • Product recovery will be at least 10% lower than that from ProbeQuant™ G-50 Micro Columns. • Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μ) following heat denaturation and phenol extraction. |
| General description: | MicroSpin™ G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme. |
| Legal Information: | MicroSpin is a trademark of Cytiva |
| Legal Information: | ProbeQuant is a trademark of Cytiva |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.) |
| UNSPSC | 41105501 |