His MultiTrap™ Fast Flow
SIGMA/GE28-4009-90 - Cytiva 28-4009-90, pack of 4 plates
Synonym: His Tag Affinity Purification
Product Type: Chemical
| average diameter | 90 μm |
| capacity | ~40 mg binding capacity(histidine-tagged protein) |
| cleaning | 2-14(Ni2+-stripped medium.) |
| manufacturer/tradename | Cytiva 28-4009-90 |
| material | polypropylene and polyethylene |
| matrix | 6% cross-linked agarose |
| packaging | pack of 4 plates |
| particle size | 45-165 μm |
| size | 127.8 mm × 85.5 mm × 30.6 mm |
| suitability | suitable for bioprocess medium |
| working range | 3-12(Ni2+-stripped medium.) |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose™ 6 Fast FLow (His MultiTrap™ FF) or Ni Sepharose™ High Performance (His MultiTrap™ HP). A protocol for His MultiTrap™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation. Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results. |
| Features and Benefits: | • Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins. • Increased convenience and consistency with prepacked 96-well plates. • No filtration needed - load unclarified sample directly, increase reproducibility, and save time. • Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum. • Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well. |
| General description: | His MultiTrap™ FF and His MultiTrap™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC). |
| Legal Information: | HisPrep is a trademark of Cytiva |
| Legal Information: | HisTrap is a trademark of Cytiva |
| Legal Information: | MultiTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 12161501 |