His Spintrap™
SIGMA/GE28-4013-53 - Cytiva 28-4013-53
Synonym: His Spintrap Column
Product Type: Chemical
| bed volume | 100 μL |
| capacity | ~750 μg binding capacity (histidine-tagged protein / column) |
| cleaning | 2-14(Ni2+ stripped medium.) |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 28-4013-53 |
| matrix | highly cross-linked 6% agarose |
| matrix active group | Nickel |
| operating pH | 3-12(Ni2+ stripped medium.) |
| packaging | pkg of 50 ea |
| parameter | 600 μL sample volume |
| particle size | ~34 μm |
| separation technique | affinity |
| storage condition | (0.15% Kathon CG) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening. • Ni Sepharose™ High Performance in prepacked spin columns. • Yields up to 750 μg pure protein per column. • Allows direct purification of both clarified and unclarified cell lysates. |
| General description: | His SpinTrap™ is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC) * See the section for licensing information in our terms and conditions. Purification of unclarified samples on His SpinTrap™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins. His SpinTrap™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap™ together with buffers prepared using His Buffer Kit. The columns are prepacked with Ni Sepharose™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose™ High Performance medium is negligible. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Legal Information: | SpinTrap is a trademark of Cytiva |
| Preparation Note: | Storage Temp.: 4°C to 30°C |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (0.15% Kathon CG) |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41106500 |