GSTrap™ 4B
SIGMA/GE28-4017-45 - Cytiva 28-4017-45, pack of 5 × 1 mL
Synonym: GST-tagged purification columns; affinity column; chromatography cartridge; glutathione Sepharose 4B; prepacked column
Product Type: Chemical
GSTrap 4B
| average diameter | 90 μm |
| bed size | 7 mm × 25 mm |
| bed volume | 1 mL |
| capacity | >25 mg binding capacity (horse liver GST/ml medium) |
| cleaning | 4-13 |
| column I.D. | 7 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| ligand | glutathione |
| manufacturer/tradename | Cytiva 28-4017-45 |
| matrix | 4% agarose |
| matrix active group | glutathione functional group |
| packaging | pack of 5 × 1 mL |
| parameter | <4 mL/min flow rate |
| 42 psi (H2O at 25°C) | |
| particle size | 45-165 μm |
| separation technique | affinity |
| storage condition | (20% Ethanol) |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 4-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System. |
| Features and Benefits: | • High binding capacity. • Mild elution conditions preserving Protein antigenicity and function. • Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity. • GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design. |
| General description: | GSTrap™ 4B columns are prepacked Glutathione Sepharose™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. |
| General description: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | FPLC is a trademark of Cytiva |
| Legal Information: | GSTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| UNSPSC | 23151816 |