GST MultiTrap™ 4B
SIGMA/GE28-4055-00 - Cytiva 28-4055-00
Synonym: GST-tagged purification system; chromatography plate; filter plate; glutathione Sepharose 4B; prepacked affinity plate
Product Type: Chemical
| average diameter | 90 μm |
| capacity | >25 mg binding capacity (horse liver GST/ml medium) |
| cleaning | 4-13 |
| ligand | glutathione |
| manufacturer/tradename | Cytiva 28-4055-00 |
| material | polypropylene and polyethylene |
| matrix | 4% agarose |
| packaging | pkg of 4 x 96-well |
| particle size | 45-165 μm |
| size | 127.8 mm × 85.5 mm × 30.6 mm |
| working range | 4-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | GST MultiTrap™ FF and GST MultiTrap™ 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins. Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap™ filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility. GST MultiTrap™ FF is prepacked with Glutathione Sepharose™ 4 Fast Flow, while GST MultiTrap™ 4B is prepacked with Glutathione Sepharose™ 4B. |
| Features and Benefits: | • 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins. • Highly reproducible well-to well and plate-to-plate results. • High purity in one step, high chemical stability. Load unclarified samples directly to the wells. • Wells are prepacked with Glutathione Sepharose™ media for convenience. • Easy and predictable scale-up using prepacked GSTrap™ FF, GSTPrep™ FF 16/10, and GSTrap™ 4B columns. |
| General description: | Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |
| General description: | GST MultiTrap™ FF and GST MultiTrap™ 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins. |
| Legal Information: | GSTPrep is a trademark of Cytiva |
| Legal Information: | GSTrap is a trademark of Cytiva |
| Legal Information: | MultiTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 12161501 |