HiTrap® MabSelect™
SIGMA/GE28-4082-56 - Cytiva 28-4082-56, pack of 5 × 5 mL
Synonym: HiTrap MabSelect; MabSelect column
Product Type: Chemical
| avg. part. size | 85 μm (d₅₀v is the median particle size of the cumulative volume distribution) |
| bed size | 16 mm × 25 mm |
| bed volume | 5 mL |
| capacity | ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time) |
| cleaning | 2-12 |
| column I.D. | 16 mm |
| ligand | recombinant protein A (E. coli) |
| manufacturer/tradename | Cytiva 28-4082-56 |
| matrix | highly cross-linked agarose |
| packaging | pack of 5 × 5 mL |
| parameter | 42 psi |
| storage condition | (20% Ehtanol) |
| (20% Ethanol) | |
| storage temp. | 2-8°C |
| suitability | suitable for bioprocess medium |
| working range | 3-10 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Lab Purification using the MabSelect™ Protein A tag for capture of antibodies. |
| Features and Benefits: | • HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification. • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity. • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification. |
| General description: | Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm. |
| General description: | HiTrap® MabSelect™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | MabSelect is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |