Ab Spintrap™
SIGMA/GE28-4083-47 - Cytiva 28-4083-47
Synonym: affinity column; antibody spin column; chromatography column; mAb spin column; polyclonal spin column; prepacked column; protein G Sepharose HP; spin column
Product Type: Chemical
| avg. part. size | 34 μm |
| bed volume | 100 μL |
| capacity | >1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.) |
| >1 mg binding capacity(IgG/column) | |
| cleaning | 2-9 |
| ligand | recombinant protein G lacking albumin-binding region |
| manufacturer/tradename | Cytiva 28-4083-47 |
| matrix | highly cross-linked 6% agarose |
| packaging | pkg of 50 × 100 μL |
| storage condition | (20% Ethanol) |
| storage temp. | 2-8°C |
| working range | 3-9 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Ab SpinTrap™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included. The spin columns are also suitable for use in antibody screening experiments. Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min. Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media. For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit. |
| Features and Benefits: | • Rapid and convenient small-scale purification of IgG. • Protein G Sepharose™ High Performance in prepacked spin columns. • No filtration or centrifugation of different crude samples necessary for antibody purification. • Classic and crosslink protocols for Protein enrichment (immunoprecipitation). • Elution conditions formatted for both LC-MS and electrophoresis analysis. • Elution conditions formatted for both LC-MS and electrophoresis analysis. |
| General description: | Ab SpinTrap™ are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Legal Information: | SpinTrap is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |