Protein A High Performance Spintrap™
SIGMA/GE28-9031-32 - Cytiva 28-9031-32, pack of 16 columns
Synonym: High Performance Spintrap; Protein A Spintrap
Product Type: Chemical
| avg. part. size | 34 μm |
| capacity | >1 mg binding capacity(human IgG well) |
| cleaning | 2-10 |
| feature | wetted part: no |
| ligand | native protein A |
| manufacturer/tradename | Cytiva 28-9031-32 |
| matrix | highly cross-linked 6% agarose |
| packaging | pack of 16 columns |
| storage condition | (20% Ethanol) |
| storage temp. | 2-8°C |
| working range | 3-9 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Protein A Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required. There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required. |
| Features and Benefits: | • For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use. • Classic and cross-link protocols provide flexibility. • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. • Fast binding kinetics and high capacity provide high yield. • Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses. • Easy scale-up with HiTrap® Protein A HP prepacked columns. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| General description: | protein A HP SpinTrap™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose™ HP. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Legal Information: | SpinTrap is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |