Protein A High Performance MultiTrap™
SIGMA/GE28-9031-33 - Cytiva 28-9031-33
Synonym: High Performance Protein A; MultiTrap Protein A
Product Type: Chemical
| bed size | 50 μL , per well |
| capacity | >10 mg binding capacity (human IgG / mL medium)(Human IgG) |
| crosslinking | cross-linked |
| form | plate (prepacked) |
| L × W × H | 127.8 mm × 85.5 mm × 30.6 mm |
| manufacturer/tradename | Cytiva 28-9031-33 |
| material | polypropylene and polyethylene plate |
| matrix | highly cross-linked 6% agarose |
| matrix active group | native protein A |
| operating pH | 3-9 |
| packaging | pkg of 4 ea |
| parameter | -0.5 bar vacuum limit |
| 700 × g max. centrifugal force | |
| particle size | ~34 μm |
| separation technique | affinity |
| storage condition | (20% Ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| well volume | 800 μL |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use. • Flexible format, with protocols for classic elution and cross-link method. • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. • Fast binding kinetics and high capacity provides high yield. • Easy scale-up with HiTrap® Protein A HP prepacked columns. • Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses. • MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| General description: | Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein. There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | MultiTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41105323 |