PD Miditrap G-25
SIGMA/GE28-9180-08 - Cytiva 28-9180-08
Synonym: G-25 Miditrap; Miditrap G-25; PD G-25 Miditrap
Product Type: Equipment/Book
| bed size | 13 mm × 26 mm |
| bed volume | 3.5 mL |
| cleaning | 2-13 |
| column I.D. | 13 mm |
| feature | wetted part: no |
| manufacturer/tradename | Cytiva 28-9180-08 |
| material | polypropylene and polyethylene resin |
| resin (Sephadex G-25) | |
| matrix | cross-linked dextran |
| packaging | pkg of 50 ea (columns) |
| particle size | >50 μm (dry) |
| 85-260 μm | |
| pore size | 5000 Da MWCO |
| sterility | non-sterile |
| storage condition | dry media |
| suitability | suitable for bioprocess medium |
| working range | 2-13 |
| working volume | 0.5—1 mL |
| Application: | Protein sample preparation. Desalting/Buffer Exchange/Cleanup. |
| Features and Benefits: | • Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr). • Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds. • High desalting capacity. • Flexible protocols with ability to use gravity flow or centrifugation. • Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation. |
| General description: | PD MidiTrap G-25 is a convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel. Can be used in a centrifugation or gravity protocol. PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide) |
| Preparation Note: | Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH) |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 41115709 |