HiScreen™ MabSelect™
SIGMA/GE28-9269-73 - Cytiva 28-9269-73
Synonym: AKTA column; affinity matrix; chromatography cartridge; mAb resin; prepacked column
Product Type: Chemical
HiScreen MabSelect
| avg. part. size | 85 μm (d₅₀v is the median particle size of the cumulative volume distribution) |
| bed size | 7.7 mm × 100 mm |
| bed volume | 4.7 mL |
| capacity | ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time) |
| cleaning | 2-12 |
| column I.D. | 7.7 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| ligand | recombinant protein A (E. coli) |
| manufacturer/tradename | Cytiva 28-9269-73 |
| matrix | highly cross-linked agarose |
| matrix active group | protein A functional group |
| packaging | pkg of 1 ea |
| parameter | 44 psi |
| separation technique | affinity |
| storage condition | (20% Ehtanol) |
| (20% Ethanol) | |
| storage temp. | 2-8°C |
| suitability | suitable for bioprocess medium |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-10 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ MabSelect™ columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production. |
| Features and Benefits: | • MabSelect™ is a BioProcess medium for capture of MAbs from large sample volumes. • Prepacked, ready-to-use columns for convenient process development. • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening. • Easily connected in series to achieve 20 cm bed height. • Small bed volume gives fast results and minimal sample/buffer consumption. • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity. |
| General description: | Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm. |
| General description: | HiScreen™ MabSelect™ is prepacked with MabSelect™ and is part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs). |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | HiScreen is a trademark of Cytiva |
| Legal Information: | MabSelect is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 13111000 |