HiScreen™ Butyl Fast Flow
SIGMA/GE28-9269-84 - Cytiva 28-9269-84
Synonym: AKTA column; HIC resin; chromatography cartridge; hydrophobic matrix; prepacked column
Product Type: Chemical
HiScreen Butyl FF
| average diameter | 90 μm |
| bed size | 7.7 mm × 100 mm |
| bed volume | 4.7 mL |
| cleaning | 2-14 |
| column I.D. | 7.7 mm |
| ligand | butyl |
| manufacturer/tradename | Cytiva 28-9269-84 |
| matrix | 4% cross-linked agarose |
| packaging | pkg of 4.7 mL |
| parameter | 1.5 bar (22 psi) (Over the Packed Bed During Operation) |
| 22 psi | |
| particle size | 45-165 μm |
| suitability | suitable for bioprocess medium |
| working range | 3-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Butyl Sepharose 4 Fast Flow is a well-established, standard aliphatic HIC medium for capture and intermediate purification. • Prepacked, ready-to-use columns for convenient process development optimization. • 1NA cm bed height of HiScreen columns is designed to allow method optimization and parameter screening. • Easily connected in series to achieve 2NA cm bed height. • Small bed volume gives fast results and minimal sample/buffer consumption. • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity. |
| General description: | HiScreen Butyl FF columns are prepacked with Butyl Sepharose 4 Fast Flow hydrophobic interaction chromatography (HIC) resins. The columns are an excellent choice for method optimization and parameter screening. The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 2NA cm. Media prepacked in HiScreen HIC columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production. |
| Legal Information: | HiScreen is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 2 to 8 °C (20% Ethanol or 0.01 M NaOH) |
| Preparation Note: | Store at 2 to 8°C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| UNSPSC | 13111000 |