HIS Spintrap™ Kit
SIGMA/GE28-9321-71 - Cytiva 28-9321-71, pkg of 1 kit (50 columns + buffers)
Synonym: HIS chromatography columns; HIS-tagged purification columns; IMAC columns; Ni Sepharose High Performance; immobilized metal ion columns; spin columns
Product Type: Chemical
Cytiva-11-0026-88
| bed volume | 100 μL |
| capacity | ~750 μg binding capacity (histidine-tagged protein / column) |
| cleaning | 2-14(Ni2+ stripped medium.) |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 28-9321-71 |
| matrix | highly cross-linked 6% agarose |
| matrix active group | nickel |
| operating pH | 3-12(Ni2+ stripped medium) |
| packaging | pkg of 1 kit (50 columns + buffers) |
| parameter | 600 μL sample volume |
| particle size | ~34 μm |
| separation technique | affinity |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening. • Ni Sepharose High Performance in prepacked spin columns. • Yields up to 750 µg pure protein per column. • Allows direct purification of both clarified and unclarified cell lysates. |
| General description: | His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). |
| Legal Information: | SpinTrap is a trademark of Cytiva |
| Other Notes: | The kit contains: 1 pack - GE28-4013-53 - His SpinTrap™ 1 pack - GE11-0034-00 - His Buffer Kit |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H314 |
| Precautionary statements | P280 - P301 + P310 + P331 - P303 + P361 + P353 + P310 - P304 + P340 + P310 - P305 - P310 - P405 - P501 |
| UNSPSC | 41106500 |
| Components | His Buffer Kit, Cytiva 11-0034-00; His Spintrap™, Cytiva 28-4013-53 |