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Dextrin Sepharose High Performance

SIGMA/GE28-9355-98 - Cytiva 28-9355-98, pack of 100 mL

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-GE28-9355-98 1 pkg
$4250.00
1/EA
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avg. part. size 34 μm
capacity ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
cleaning in place 2-13
ligand dextrin
manufacturer/tradename Cytiva 28-9355-98
matrix highly cross-linked 6% agarose
packaging pack of 100 mL
storage condition (20% Ehtanol)
storage temp. 2-8°C
working range >7
Analysis Note: To view the Certificate of Analysis for this product, please visit www.cytiva.com .
Application: Dextrin Sepharose High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.
Features and Benefits: • Dextrin Sepharose High Performance is a robust, high resolution medium based on the 34 μm Sepharose High Performance matrix
• The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
• Physiological conditions and mild elution preserve target Protein activity
• Dextrin Sepharose High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications
General description: Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-ßGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).
General description: Dextrin Sepharose High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).
Legal Information: Sepharose is a trademark of Cytiva
Preparation Note: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Storage Temp. 2-8°C
UNSPSC 41106500

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