HiPrep™ Heparin Fast Flow 16/10
SIGMA/GE28-9365-49 - Cytiva 28-9365-49
Synonym: Heparin affinity chromatography
Product Type: Chemical
HiPrep Heparin FF 16/10
| average diameter | 90 μm |
| bed size | 16 mm × 100 mm |
| bed volume | 20 mL |
| cleaning | 4-13 |
| column I.D. | 16 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| ligand | porcine heparin |
| manufacturer/tradename | Cytiva 28-9365-49 |
| matrix | 6% cross-linked agarose |
| matrix active group | heparin |
| packaging | pkg of 1 ea |
| parameter | 1.5 bar (22 psi) (Over the Packed Bed During Operation) |
| 2 ml/min - 10 ml/min flow rate | |
| 22 psi | |
| particle size | 45-165 μm |
| separation technique | affinity |
| storage condition | (20% ethanol containing 0.05 M sodium acetate) |
| suitability | suitable for bioprocess medium |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 4-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | The column provides fast, preparative separations of Proteins and other biomolecules. Immobilized heparin has two main modes of interaction with Proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma Proteins, lipoProteins, Protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to Sepharose™ 6 Fast FLow with a chemically optimized linkage. Heparin is a mucopolysaccharide consisting of a repeating dimer of α-L-dipyranuronic acid 2-sulfate, and 2-deoxy-2-sulfamino-α-D-g |
| Features and Benefits: | • 20 mL column optimized for convenient scale-up purification . • Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma Proteins. • Simple operation with single pump configurations, as well as ÄKTA® design systems. • Excellent flow characteristics. |
| General description: | HiPrep™ Heparin FF 16/10 is prepacked with Heparin Sepharose™ 6 Fast FLow for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | HiPrep is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (0.05 M Sodium Acetate containing 20% Ethanol) |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 13111000 |