HiScreen™ Ni Fast Flow
SIGMA/GE28-9782-44 - Cytiva 28-9782-44
Product Type: Chemical
HiScreen Ni FF
| average diameter | 90 μm |
| bed size | 7.7 mm × 100 mm |
| bed volume | 4.7 mL |
| capacity | ~40 mg binding capacity(histidine-tagged protein) |
| cleaning | 2-14(Ni2+-stripped medium.) |
| column I.D. | 7.7 mm |
| manufacturer/tradename | Cytiva 28-9782-44 |
| matrix | 6% cross-linked agarose |
| packaging | pkg of 1 ea |
| parameter | 1.5 bar (22 psi) (Over the Packed Bed During Operation) |
| 22 psi | |
| particle size | 45-165 μm |
| suitability | suitable for bioprocess medium |
| working range | 3-12(Ni2+-stripped medium.) |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production. HiScreen™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose™ 6 Fast FLow which has high binding capacities for histidine-tagged Proteins and minimal Ni2+ leakage. |
| Features and Benefits: | • High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+. • Prepacked, ready-to-use columns for convenient process development. • Excellent choice for method optimization and parameter screening due to the 10 cm bed height. • Easy connection in series to achieve 20 cm bed height. • Small bed volume gives fast results and minimal sample/buffer consumption • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity |
| General description: | HiScreen™ columns prepacked with Ni Sepharose™ 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC). |
| Legal Information: | HiScreen is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 13111000 |