Superdex® 200 Increase 10/300 GL
SIGMA/GE28-9909-44 - Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size
Synonym: Superdex Increase
Product Type: Chemical
| bed volume | ~24 mL |
| cleaning | 1-14 |
| column L × I.D. | 30 cm × 10 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 28-9909-44 |
| material | glass column |
| polyethylene filter | |
| matrix | cross-linked agarose-dextran composite |
| matrix active group | dextran-agarose |
| mol wt | Mp 1-100 kDa (dextrans fractionation range) |
| protein Mr 1.3 MDa (exclusion limit) | |
| protein Mr 10-600 kDa (fractionation range) | |
| operating pH | 3—12 |
| packaging | pkg of 1 ea |
| parameter | 0.75 mL/min flow rate |
| 1.8 mL/min max. flow rate | |
| 25-500 μL sample volume | |
| 3.0 Mpa pressure drop required | |
| particle size | 8.6 μm |
| product line | Superdex® |
| separation technique | size exclusion (SEC) |
| shipped in | ambient |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| Application: | For both preparative and analytical purposes |
| Application: | Gel filtration for small-scale (25-500 mL) preparative purification. |
| Application: | Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride |
| Features and Benefits: | • Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200. • Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity. • High flow rates: rigid beads give excellent pressure/flow properties. |
| General description: | Superdex® 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000. Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times. Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions. Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate. This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples. |
| Legal Information: | Superdex is a registered trademark of Cytiva |
| Other Notes: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| Preparation Note: | Storage Temp.: 4°C to 30°C |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NA 1993 / PGIII |
| Storage Temp. | 2-30°C |
| UNSPSC | 41115700 |