HiScreen™ Capto™ Butyl
SIGMA/GE28-9924-73 - Cytiva 28-9924-73, pack of 1 column
Product Type: Chemical
HiScreen Capto Butyl
| avg. part. size | 75 μm (d50v) |
| bed size | 7.7 mm × 100 mm |
| capacity | 27 mg binding capacity(27 mg BSA /ml (at QB10%)) |
| cleaning | 3-14 |
| column I.D. | 7.7 mm |
| ligand | butyl |
| manufacturer/tradename | Cytiva 28-9924-73 |
| matrix | highly cross-linked agarose |
| packaging | pack of 1 column |
| suitability | suitable for bioprocess medium |
| working range | 3-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production. Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration. |
| Features and Benefits: | • Prepacked with Capto™ Butyl • Excellent for method optimization and parameter screening due to the 10 cm bed height • Easily connected in series to achieve 20 cm bed height • Small bed volume for fast results and minimal sample/buffer consumption • Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity |
| General description: | Capto™ Phenyl (High sub) and Capto™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification. |
| Legal Information: | Capto is a trademark of Cytiva |
| Legal Information: | HiScreen is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 23151817 |