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His Spintrap™ TALON®

SIGMA/GE29-0005-93 - Cytiva 29-0005-93

Synonym: HIS spin columns; HIS-tagged purification columns; IMAC columns; TALON Superflow cobalt-IMAC; immobilized metal ion chromatography columns

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-GE29-0005-93 1 pkg
 $653.00
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His SpinTrap TALON®

 

bed volume 100 μL
capacity ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
  ≤1 mg binding capacity(histidine-tagged)
cleaning 2-14(Co2+-stripped medium)
ligand TALON® Superflow Cobalt-IMAC
manufacturer/tradename Cytiva 29-0005-93
matrix 6% cross-linked agarose
packaging pkg of 50 x 100 μL
particle size 60-160 μm
storage temp. 2-8°C
working range 3-12(Co2+-stripped medium)
Analysis Note: To view the Certificate of Analysis for this product, please visit www.cytiva.com .
Application: His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.
Features and Benefits: • Suitable for purification of multiple samples in parallel, such as screening.
• Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
• With a microcentrifuge, one run takes about 10 minutes.
General description: His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.
Legal Information: SpinTrap is a trademark of Cytiva
Legal Information: Talon is a registered trademark of Clontech
Preparation Note: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Storage Temp. 2-8°C
UNSPSC 41106500

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