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His GraviTrap™ TALON®

SIGMA/GE29-0005-94 - Cytiva 29-0005-94, pack of 10 columns

Synonym: His GraviTrap

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-GE29-0005-94 1 pkg
 $412.00
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bed volume 1 mL
capacity ≤15 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
  ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
cleaning 2-14(Co2+-stripped medium)
column I.D. 15 mm
ligand TALON® Superflow Cobalt-IMAC
manufacturer/tradename Cytiva 29-0005-94
matrix 6% cross-linked agarose
packaging pack of 10 columns
particle size 60-160 μm
storage temp. 2-8°C
working range 3-12(Co2+-stripped medium)
Analysis Note: To view the Certificate of Analysis for this product, please visit www.cytiva.com .
Application: His GraviTrap™ Talon® gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged Proteins directly from clarified or unclarified cell lysates. His GraviTrap™ Talon® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification.

Large sample volumes can be applied in one go, and the histidine-tagged Protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 ml. Leakage of cobalt ions from Talon® Superflow medium is negligible.
Features and Benefits: • Convenient histidine-tagged purification with high purity (>90%) and binding capacity of up to 15 mg/column.
• Ready-to-use gravity flow column prepacked with 1 mL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
• One run takes about 30 minutes, and large sample volumes can be applied all at once.
General description: His GraviTrap™ Talon® is a prepacked gravity f low column for convenient manual purification of histidine-tagged proteins.
Legal Information: GraviTrap is a trademark of Cytiva
Legal Information: Talon is a registered trademark of Clontech
Preparation Note: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Storage Temp. 2-8°C
UNSPSC 23151816

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800-334-7725
office@krackeler.com


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