HiTrap® Protein G HP
SIGMA/GE29-0485-81 - Cytiva 29-0485-81, pack of 1 mL
Synonym: affinity column; antibody purification column; chromatography column; prepacked column; protein G column
Product Type: Chemical
HiTrap Protein G HP
| availability | not available in North America |
| avg. part. size | 34 μm |
| bed size | 7 mm × 25 mm |
| bed volume | 1 mL |
| capacity | >1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.) |
| 25 mg binding capacity(human IgG/column) | |
| cleaning | 2-9 |
| column I.D. | 7 mm |
| ligand | recombinant protein G lacking albumin-binding region |
| manufacturer/tradename | Cytiva 29-0485-81 |
| matrix | highly cross-linked 6% agarose |
| packaging | pack of 1 mL |
| parameter | flow rate |
| 42 psi | |
| storage condition | (20% Ehtanol) |
| (20% Ethanol) | |
| storage temp. | 2-8°C |
| working range | 3-9 |
| Features and Benefits: | High binding capacity of 25 mg IgG/ml medium and fast binding kinetics. 34 μm bead size for high performance, high-resolution purifications. Binds broad range of different species and subclasses of IgG. Simple and proven method giving reproducible results. Designed for use with syringe, peristalic pump, or chromatography system. |
| General description: | HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NA 1993 / PGIII |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |