HisTrap™ Excel
SIGMA/GE29-0485-86 - Cytiva 29-0485-86, pack of 1 mL
Synonym: HIS chromatography column; HIS-tagged purification columns; IMAC column; Ni Sepharose excel; immobilized metal ion chromatography column; prepacked column
Product Type: Chemical
HisTrap excel (1 mL and 5 mL)
| average diameter | 90 μm |
| bed volume | 1 mL |
| capacity | ≥10 mg binding capacity (histidine-tagged protein / mL sedimented medium) |
| cleaning | 2-14 |
| column I.D. × L | 7 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 29-0485-86 |
| matrix | highly cross-linked agarose, spherical |
| matrix active group | Nickel |
| packaging | pack of 1 mL |
| parameter | 1-4 mL/min flow rate |
| 5 bar max. pressure | |
| separation technique | affinity |
| shipped in | ambient |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 2-12 |
| Features and Benefits: | • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity • Increase target protein yield and decrease degradation through reduced and simplified sample handling • HisTrap excel columns allow direct purification of cell-free, unclarified samples • HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet. • HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled. |
| General description: | Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function. |
| General description: | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins. |
| Legal Information: | HisTrap is a trademark of Cytiva |
| Other Notes: | H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NA 1993 / PGIII |
| Storage Temp. | 2-30°C |
| UNSPSC | 23151816 |