GSTrap™ 4B
SIGMA/GE29-0486-09 - Cytiva 29-0486-09, pack of 1 mL
Synonym: GST-tagged purification columns; affinity column; chromatography cartridge; glutathione Sepharose 4B; prepacked column
Product Type: Chemical
| availability | not available in North America |
| average diameter | 90 μm |
| bed size | 7 mm × 25 mm |
| bed volume | 1 mL |
| capacity | >25 mg binding capacity (horse liver GST/ml medium)(H2O at 25°C) |
| cleaning | 4-13 |
| column I.D. | 7 mm |
| ligand | glutathione |
| manufacturer/tradename | Cytiva 29-0486-09 |
| matrix | 4% agarose |
| packaging | pack of 1 mL |
| parameter | 42 psi (H2O at 25°C) |
| particle size | 45-165 μm |
| working range | 4-13 |
| Application: | Tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC System. |
| Features and Benefits: | • High binding capacity. • Mild elution conditions preserving protein antigenicity and function. • Easy one-step purifications of Glutathione S-Transferase (GST) tagged proteins resulting in high purity. • GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as AKTA design. |
| General description: | GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. |
| Legal Information: | GSTrap is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NA 1993 / PGIII |
| UNSPSC | 23151817 |