MBPTrap™ HP
SIGMA/GE29-0486-41 - Cytiva 29-0486-41, pack of 1 mL
Synonym: MBP-tagged purification columns; affinity column; chromatography cartridge; column; dextrin Sepharose HP; prepacked column
Product Type: Chemical
| avg. part. size | 34 μm |
| bed volume | 1 mL |
| capacity | ~16 mg binding capacity (MBP2*-b-galactosidase / mL medium) |
| ~7 mg binding capacity (MBP2*-paramyosin-d-Sal / mL medium) | |
| cleaning | 2-13 |
| column I.D. × L | 7 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 29-0486-41 |
| matrix | highly cross-linked 6% agarose |
| matrix active group | dextrin |
| operating pH | >7 |
| packaging | pack of 1 mL |
| parameter | 0.3 Mpa max. pressure |
| 1 mL/min flow rate | |
| 4 mL/min max. flow rate | |
| separation technique | affinity |
| storage condition | (20% ethanol) |
| storage temp. | 2-8°C |
| technique(s) | liquid chromatography (LC): suitable |
| Features and Benefits: | • HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium. • Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH. • Physiological conditions and mild elution preserve the activity of the target protein. • Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag. |
| General description: | MBPTrap™ HP columns prepacked with Dextrin Sepharose™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps. Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH. |
| General description: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Legal Information: | MBPTrap is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NA 1993 / PGIII |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151817 |