HiTrap™ Protein L
SIGMA/GE29-0486-65 - Cytiva 29-0486-65, pack of 1 mL
Synonym: Protein L chromatography
Product Type: Chemical
HiTrap Protein L (1 and 5 mL)
| availability | not available in North America |
| avg. part. size | 85 μm (d₅₀v is the median particle size of the cumulative volume distribution) |
| column I.D. | 7 mm |
| ligand | recombinant protein L (E. coli), mammalian free |
| manufacturer/tradename | Cytiva 29-0486-65 |
| matrix | rigid, highly cross-linked agarose |
| packaging | pack of 1 mL |
| parameter | 5 bar |
| storage condition | (20% Ehtanol) |
| storage temp. | 2-8°C |
| suitability | suitable for bioprocess medium |
| working range | 2-10(pH interval where the medium can be operated without significant change in function.) |
| Application: | Affinity chromatoghraphy; capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs. |
| Features and Benefits: | • Suitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (dAbs), and single-chain variable fragments (scFvs). • Enables a platform approach to antibody fragment purification. • High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin. • Convenient for process development, as well as small-scale purification. • HiTrap™ format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system. |
| General description: | HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains. |
| Legal Information: | HisTrap is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NA 1993 / PGIII |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |