HiTrap® MabSelect SuRe™
SIGMA/GE29-0491-04 - Cytiva 29-0491-04, pack of 1 mL
Synonym: affinity column; chromatography column; mAb purification column; prepacked column; protein A column
Product Type: Chemical
| avg. part. size | ~85 μm |
| bed volume | 1 mL |
| capacity | ~35 mg binding capacity (human lgG / ml resin) |
| cleaning | 3-13.7 |
| column I.D. × L | 7 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 29-0491-04 |
| matrix | highly cross-linked agarose |
| matrix active group | alkali-stabilized protein A-derived (E. coli) |
| packaging | pack of 1 mL |
| parameter | 0.5 mL/min flow rate (recommended) |
| 4 mL/min max. flow rate | |
| 5 bar max. pressure | |
| separation technique | affinity |
| storage condition | (20% Ethanol) |
| storage temp. | 2-8°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12 |
| Features and Benefits: | • Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH. • Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage. • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity. • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification. • Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs. |
| General description: | Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm. |
| General description: | HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP). |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | Free MabSelect SuRe Ligand "MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | MabSelect SuRe is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NA 1993 / PGIII |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |