HisTrap™ High Performance
SIGMA/GE29-0510-21 - Cytiva 29-0510-21, pack of 1 mL
Synonym: HIS chromatography column; HIS-tagged purification columns; IMAC column; Ni Sepharose HP; immobilized metal ion chromatography column; prepacked column
Product Type: Chemical
| avg. part. size | 34 μm |
| bed volume | 1 mL |
| capacity | ≥40 mg binding capacity (histidine-tagged protein / ml medium) |
| cleaning | 2-14(Ni2+-stripped medium.) |
| column I.D. × L | 7 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 29-0510-21 |
| matrix | highly cross-linked 6% agarose |
| matrix active group | Ni2+ |
| packaging | pack of 1 mL |
| parameter | 5 bar max. pressure |
| 5 mL/min flow rate (recommended) | |
| separation technique | affinity |
| shipped in | ambient |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12(Ni2+-stripped medium) |
| Application: | HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications. |
| Features and Benefits: | • High binding capacity, at least 40 mg/ml chromatography medium. • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives. • Negligible Ni2+ leakage • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design |
| General description: | HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). |
| Legal Information: | HisTrap is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NA 1993 / PGIII |
| Storage Temp. | 2-30°C |
| UNSPSC | 23151816 |