Superose® 6 Increase 10/300 GL
SIGMA/GE29-0915-96 - Cytiva 29-0915-96, 10/300 GL
Synonym: Superose® 6 Increase
Product Type: Chemical
29091596 Stock Photo
| bed volume | 24 mL |
| cleaning | 1-14 |
| crosslinking | cross-linked |
| form | prepacked column |
| L × I.D. | 300 mm × 10 mm |
| manufacturer/tradename | Cytiva 29-0915-96 |
| material | glass column |
| polyethylene filter | |
| matrix | cross-linked agarose |
| matrix active group | agarose |
| mol wt | Mp 1-300 kDa (dextrans fractionation range) |
| protein Mr 40 MDa (exclusion limit) | |
| protein Mr 5-5000 kDa (fractionation range) | |
| packaging | package of 1 ea |
| parameter | 0.5 mL/min flow rate (recommended) |
| 1.50 mL/min max. flow rate | |
| 25-500 μL sample volume | |
| 3 Mpa pressure drop required | |
| particle size | ~ 8.6 μm |
| product line | Superose® |
| separation technique | size exclusion (SEC) |
| shipped in | ambient |
| storage condition | (20% ethanol ) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place) |
| Application: | Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions. |
| Features and Benefits: | • Increased resolution compared with Superose 6, for higher purity and improved analysis results. • Reduced runtime compared with Superose 6, for rapid results. • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes. • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over. |
| General description: | Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. |
| Legal Information: | Superdex is a registered trademark of Cytiva |
| Legal Information: | Superose is a registered trademark of Cytiva |
| Other Notes: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| Preparation Note: | Storage Temp.: 4°C to 30°C |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41115700 |