Superdex® Increase 75
SIGMA/GE29-1487-23 - 3.2/300 GL, Cytiva 29-1487-23, column L × I.D. 30 cm × 3.2 mm, 9 μm avg. part. size
Synonym: Superdex 75 increase
Product Type: Chemical
29148721 Stock Photo
| avg. part. size | 9 μm |
| bed volume | ~2.4 mL |
| cleaning in place | 1-14 |
| column L × I.D. | 30 cm × 3.2 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 29-1487-23 |
| material | glass column |
| titanium frit | |
| matrix | Composite of cross-linked agarose and dextran |
| matrix active group | agarose-dextran |
| mol wt | 3000-70,000 Da (globular proteins) |
| Mp 0.5-30 kDa (dextrans fractionation range) | |
| protein Mr 100 kDa (exclusion limit) | |
| protein Mr 3-70 kDa (fractionation range) | |
| operating pH range | 3-12 |
| packaging | pkg of 1 ea |
| parameter | 0.075 mL/min flow rate (Recommended ) |
| 0.15 mL/min max. flow rate | |
| 2000 kPa pressure drop required | |
| 4-50 μL sample volume | |
| product line | Superdex® |
| separation technique | size exclusion (SEC) |
| shelf life | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| shipped in | ambient |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12 |
| Features and Benefits: | Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity, Superdex 75 Increase columns offer: • Up to 50% higher resolution compared with predecessor Superdex 75, for improved purity and analysis results. • Runtime reduced down to one third compared with predecessor Superdex 75, with maintained resolution for results faster. • Versatile use in both preparative and analytical applications, especially with recombinant tagged proteins. • Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover. • Higher lot-to-lot consistency compared with predecessor Superdex 75. |
| General description: | Our new generation columns called "Increase" offer higher resolution and shorter run times than their predecessors. Superdex 75 Increase is designed for small-scale preparative purification and analysis of recombinant tagged or other proteins with molecular weights from 3000 up to 70 000, such as recombinant tagged proteins |
| Legal Information: | Superdex is a registered trademark of Cytiva |
| Other Notes: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| Preparation Note: | Store at 4-30 °C (20% ethanol) |
| Symbol |  GHS02,GHS02 |
| Signal word | Warning-Warning |
| Hazard statements | H226 - H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P210 - P233 - P240 - P241 - P242 - P243 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | 104.9 °F |
| Flash Point(C) | 40.5 °C |
| Storage Temp. | 2-30°C |
| UNSPSC | 41115711 |