Exonuclease I, 10 U/μL
SIGMA/GEE70073X - Cytiva E70073X, pack of 5000 units
Synonym: ExoI; Exonuclease I Enzyme; PCR cleanup enzyme; single-stranded DNA exonuclease
Product Type: Chemical
Cytiva-27-7007-34
| biological source | Escherichia coli |
| manufacturer/tradename | Cytiva E70073X |
| packaging | pack of 5000 units |
| shipped in | dry ice |
| storage temp. | −20°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Exonuclease I has been used to quantify the expression of 56 genes in each F2 XY gonad. |
| Biochem/physiol Actions: | Exonuclease I (Exo I) from E. coli can degrade single-stranded DNA (ssDNA) from 3′ to 5′. It participates in various DNA repair systems, like, RecBCD-dependent homologous recombination, methyl-dependent mismatch repair and genomic tandem repeat maintenance. |
| Features and Benefits: | • Elimination of residual single-stranded DNA containing a 3′-terminus. • Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA. • Measuring DNA helicase activity. |
| General description: | Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides. |
| General description: | Exonuclease I (Exo I) from E. coli is an Mg2+ ion dependent exonuclease. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |