Exonuclease I
SIGMA/GEE70073Z - Cytiva E70073Z, pack of 2500 U
Synonym: Exo I; PCR cleanup reagent; catalyst; enzyme; nuclease; protein
Product Type: Chemical
Cytiva-27-7007-34
| manufacturer/tradename | Cytiva E70073Z |
| packaging | pack of 2500 U |
| shipped in | dry ice |
| storage temp. | −20°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides. |
| Biochem/physiol Actions: | Exonuclease I (EXO1) maintains genomic stability by participating in DNA mismatch repair system, DNA recombination, DNA replication and DNA double-stranded break repair in eukaryotes. It also modulates meiosis, immunoglobulin maturation, and micro-mediated end-joining. It functions on DNA with blunt, nicked, gapped and 3′-overhangs. It also possesses endonuclease action and removes 5′-single-stranded flaps that protrudes from a double stranded DNA. Mutation in EXO1 gene results in increased susceptibility to develop cancer. |
| Features and Benefits: | • Eliminating residual single-stranded DNA containing a 3′-terminus. • Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA. • Measuring DNA helicase activity. |
| General description: | Exonuclease I. |
| General description: | The EXO1 (exonuclease I) gene is mapped to human chromosome 1q43 and codes for a 5′→3′ exonuclease of 846 amino acids. It belongs to the 5′-nuclease superfamily. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |