Shrimp Alkaline Phosphatase
SIGMA/GEE70092Y - Cytiva E70092Y, pack of 500 U
Synonym: AP; PCR buffer component; dephosphorlyation catalyst; endonuclease buffer ingredient; enzyme; phosphate hydrolase; protein
Product Type: Chemical
Cytiva-25-0039-33
| biological source | shrimp |
| form | liquid |
| manufacturer/tradename | Cytiva E70092Y |
| mol wt | 155 kDa |
| packaging | pack of 500 U |
| shipped in | dry ice |
| storage temp. | −20°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Biochem/physiol Actions: | Shrimp alkaline phosphatase (SAP) catalyzes the in vitro dephosphorylation of DNA or deoxynucleotides (dNTPs). The enzyme activity can be completely inhibited by ethylenediaminetetraaceti |
| Biochem/physiol Actions: | Shrimp alkaline phosphatase (SAP) catalyzes the in vitro dephosphorylation of DNA or deoxynucleotides (dNTPs). The enzyme activity can be completely inhibited by ethylenediaminetetraaceti |
| Features and Benefits: | • Removing 5′-phosphates from DNA and RNA. • Easily inactivated by heat. |
| General description: | Shrimp alkaline phosphatase (SAP) has a high specific activity and is a heat-labile alkaline phosphatase. It can be inactivated by a short rise in temperature to 65 °C. SAP is a 155,000Da homodimeric protein with dimensions of about 95 Å×65 Å×50 Å and mol. wt of 155,000. Completely and irreversibly inactivated in Tris buffers at pH 8.0-8.5 by heating for 15 min at 65°C. No further treatment is necessary. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |