ECL™ Western Blotting Analysis System
SIGMA/GERPN2108 - Cytiva RPN2108
Synonym: RPN2108; chemiluminescent workflow; kit; reagents; western blotting detection
Product Type: Chemical
Cytiva-25-0062-64
| description | Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White |
| feature | wetted part: no |
| manufacturer/tradename | Cytiva RPN2108 |
| packaging | pkg of 1 kit |
| storage temp. | 2-8°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | ECL™ Blotting Analysis System has been used in western blotting. |
| Application: | Western Blotting |
| Features and Benefits: | • Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL™ reagents. • Optimized system provides rapid, sensitive results using simple protocols. • Designed for use with nitrocellulose and PVDF membranes. • High sensitivity: Up to 10 times more sensitive than colorimetric methods. • Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods. • Easy reprobing: Save time and materials by reprobing on membranes up to 10 times. • Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL, • Non-radioactive-no specialized facilities needed • Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide |
| General description: | ECL™ Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system. ECL™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera. Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane: Anti-mouse HRP conjugate, 100 μl Anti-rabbit HRP conjugate, 100 μl ECL™ Detection Reagents 1 and 2, 62.5 mL each Blocking agent, 5 g. |
| Legal Information: | ECL is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P101 - P102 - P103 - P261 - P272 - P280 - P302 + P352 - P333 + P313 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41105332 |