ECL™ Prime Western Blotting System
SIGMA/GERPN2232 - Cytiva RPN2232
Synonym: Amersham™ ECL™ Prime, Chemiluminescent Western blotting kit, chemiluminescent detection reagent; Cytiva ECL kit; ECL detection reagents; HRP substrates; RPN2232; Western blot substrates; chemiluminescent substrates; luminol peroxide reagents
Product Type: Chemical
| color | Detection reagent 1: Colorless Detection reagent 2: Colorless |
| manufacturer/tradename | Cytiva RPN2232 |
| packaging | pkg of 1 kit |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Sensitive quantitative or confirmatory Western Blotting |
| Features and Benefits: | • High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity. • Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection. • Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham™ Hyperfilm. • Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers. • Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond-ECL™ (nitrocellulose) membranes. • Recommended membrane Hybond-P or Hybon ECL™ |
| General description: | Amersham™ ECL™ Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents. Amersham™ ECL™ Prime consolidates and builds on the benefits of Amersham™ ECL™ Plus and Amersham™ ECL™ Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. |
| Legal Information: | Amersham is a trademark of Cytiva |
| Legal Information: | ECL is a trademark of Cytiva |
| Legal Information: | ECL Prime This product or portions thereof is manufactured and sold under license from Cyanagen Srl and is subject of US patent 7,855,287, US Patent 7,803,573, and Italian application number TO2010A000580, together with other equivalent granted patents and patent applications in other countries. |
| Legal Information: | Rainbow is a trademark of Cytiva |
| Other Notes: | Color: Detection reagent 1: colorless Detection reagent 2: colorless Sensitivity: ~1 pg |
| Other Notes: | The following components are included in the Amersham ECL Prime detection reagent kit. • RPN2232 Solution A: Luminol solution, 50 ml Solution B: Peroxide solution, 50 ml sufficient for 1000 cm2 membrane |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41105332 |