ECL™ Prime Western Blotting Detection Reagent
SIGMA/GERPN2236 - Cytiva RPN2236
Synonym: HRP substrates; Western blot substrates; chemiluminescent detection reagent, Western blotting detection reagent; chemiluminescent substrates; luminol peroxide reagents
Product Type: Chemical
| manufacturer/tradename | Cytiva RPN2236 |
| packaging | pkg of 1 kit |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | ECL™ Prime Western blotting detection reagent has been used to detect chemiluminescent signals during Western blotting. |
| Features and Benefits: | • Compatible with Rainbow Molecular Weight Markers and Amersham™ ECL DualView™ Western Blotting Markers. • High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity. • Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection. • Optimized for use with Amersham Hybond®-P (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes. • Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm™. |
| General description: | Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents. Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. |
| Legal Information: | Amersham is a trademark of Cytiva |
| Legal Information: | ECL DualView is a trademark of Amersham |
| Legal Information: | ECL is a trademark of Cytiva |
| Legal Information: | Hybond is a registered trademark of Cytiva |
| Legal Information: | Hyperfilm is a trademark of Cytiva |
| Other Notes: | The following components are included in the Amersham ECL Prime detection reagent kit. • RPN2236 Solution A: Luminol solution, 3 × 50 ml Solution B: Peroxide solution, 3 × 50 ml sufficient for 3000 cm2 membrane |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41105332 |