ECL™ Advance Blocking Reagent
SIGMA/GERPN418 - Cytiva RPN418
Synonym: ECL™ Blocking Agent; Western blotting reagent; blocker; buffer; membrane blocker
Product Type: Chemical
| color | Yellow. Pale color |
| feature | wetted part: no |
| manufacturer/tradename | Cytiva RPN418 |
| packaging | pkg of 40 g |
| storage temp. | 2-8°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | ECL™ Advance Blocking Reagent may be used for high-sensitivity ECL and fluorescence western blotting applications. |
| General description: | During the Western blotting process, non-specific binding of antibodies to the membrane is detrimental to the specificity and sensitivity of the assay. To overcome this challenge, it is essential to block parts of the membrane not already occupied by proteins. The Amersham™ ECL™ Prime blocking agent can be used for blocking Amersham Protran nitrocellulose (NC) and Amersham Hybond polyvinylidene fluoride (PVDF) membranes when used along with the ECL™ chemiluminescence detection reagents, such as Amersham ECL™, ECL™ start, ECL™ Prime, ECL™ Select, and ECL™ Plex. Each pack contains 40 g of blocking agent, which is sufficient for at least 20 mini blots. |
| Legal Information: | Amersham is a trademark of Cytiva |
| Legal Information: | ECL is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41105332 |