Vistra ECF™ Substrate
SIGMA/GERPN5785 - Cytiva RPN5785
Synonym: alkaline phosphatase fluoresence; cytiva RPN5785; detector; fluorescent substrate; fluorophore; reagent
Product Type: Chemical
Cytiva-11-0026-88
| color | ECF substrate: Off-white/white to yellowish ECF substrate dilution buffer: Colorless |
| manufacturer/tradename | Cytiva RPN5785 |
| packaging | pkg of 1 kit |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Vistra ECF™ substrate has been used in Western blotting. |
| Features and Benefits: | • A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm) • For use in western blotting, dot, and slot blotting applications • Sensitivity comparable to chemiluminescence • The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan • Eliminates need for darkroom and film |
| General description: | Alkaline phosphatase dephosphorylates ECF™ Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength. The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer. |
| Legal Information: | ECF is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H318 - H373 |
| Precautionary statements | P260 - P280 - P305 - P310 - P314 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41105332 |