Golgi Isolation Kit
SIGMA/GL0010 - sufficient for 50 g (tissue)
Synonym: Golgi Kit; Isolation Kit for Golgi
Product Type: Chemical
The Golgi-enriched fraction was isolated from rat kidney using the Golgi Isolation Kit. 15 μg of total protein from each step were separated on a 7.5% SDS-PAGE gel: Lane 1: homogenate Lane 2: 3000 × g supernatant Lane 3: Golgi gradient-enriched fraction
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | fractionation: suitable |
| usage | sufficient for 50 g (tissue) |
| Analysis Note: | The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart. |
| Application: | Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient. |
| General description: | The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100). |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL |