Glutathione Reductase Assay Kit
SIGMA/GRSA - Sufficient for 100 colorimetric tests
Synonym: Glutathione Reductase Activity Assay Kit
Product Type: Chemical
Assay of Purified Glutathione Reductase. Glutathione reductase was diluted with dilution buffer to 1.4 units/ml. The dilution was assayed with both the UV and colorimetric assays provided wiith the Glutathione Reductase Assay Kit (GR-SA).
Assay of Yeast Lysate. The activity of glutathione reductase in Saccharomyces cerevisiae cell lysate (8.8 mg protein/ml) was assayed with both the UV and colorimetric assays provided with the Glutathione Reductase Assay Kit (GR-SA).
| detection method | colorimetric |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| usage | sufficient for 100 tests |
| Analysis Note: | UV assay: One unit will cause the oxidation of 1.0 μmole of NADPH at 25 °C at pH 7.5. Colorimetric assay: One unit will cause the reduction of 1.0 μmole of DTNB to TNB at 25 °C at pH 7.5. |
| Application: | Glutathione Reductase Assay Kit has been used to measure the activity of glutathione reductase as a part of oxidative stress assessment and also to study the effects of antifouling biocides on it. |
| Application: | Suitable for the measurement of glutathione reductase activity in biological samples |
| Biochem/physiol Actions: | Glutathione reductase (EC 1.6.4.2) (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid). |
| Biochem/physiol Actions: | This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid). |
| Biochem/physiol Actions: | This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid). |
| General description: | Glutathione reductase (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161503 |
| Components | 5,5′-Dithiobis(2-nitrobenzoic acid) 50 mg; Glutathione, oxidized, disodium salt 100 mg; β-Nicotinamide adenine dinucleotide 25 mg |