β-Glucuronidase Reporter Gene Staining Kit
SIGMA/GUSS
Synonym: β-Glucuronidase Staining Kit; Reporter Gene Kit; Staining Kit for Glucuronidase
Product Type: Chemical
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| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | microbe id | staining: suitable |
| usage | kit sufficient for 100 histochemical assays |
| Application: | The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins. Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells. |
| Features and Benefits: | • Histochemical staining of plant tissues expressing the E. coli GUS enzyme • Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability • GUS does not interfere with plant cell function or viability |
| General description: | Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants. |
| Other Notes: | The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indol |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |
| Components | 2× Fixation Buffer 25 mL; 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial; Reagent A for gus staining kit 50 mL; Reagent B for gus staining kit 200 μL; Reagent C for gus staining kit 200 μL |