HT Media Supplement (50×) Hybri-Max™
SIGMA/H0137 - lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma
Synonym: HT Supplement; Hypoxanthine-
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| form | lyophilized powder |
| impurities | endotoxin, tested |
| product line | BioXtra |
| Quality Level | 200  |
| sterility | γ-irradiated |
| storage temp. | −20°C |
| technique(s) | cell culture | hybridoma: suitable |
| Application: | HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max™ has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells. |
| Legal Information: | Hybri-Max is a trademark of Sigma-Aldrich Co. LLC |
| Preparation Note: | Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |