HIV-1 gag
SIGMA/H0160 - ≥95% (SDS-PAGE), recombinant, expressed in E. coli, Galactosidase-tagged
Synonym: p24 HIV antigen
MDL Number: MFCD01635027
Product Type: Chemical
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| assay | ≥95% (SDS-PAGE) |
| concentration | 1 mg/mL |
| mol wt | 39 kDa (plus 114 kDa β-Gal tag) |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| UniProt accession no. | P04591  |
| Application: | HIV-1 gag has been used in the coculture with an autologous cluster of differentiation T (CD4+ T) to study the ability of monocyte-derived dendritic cells (MDC1) to induce human immunodeficiency virus 1 (HIV−1) latency reversal (LR). |
| Biochem/physiol Actions: | Positive control for HIV antibodies. |
| Biochem/physiol Actions: | The human immunodeficiency virus 1 (HIV−1) gag (p24) antigen reacts strongly with HIV positive serum and may be used as a positive control for evaluating antibodies to HIV. At transcription sites, HIV-1 Gag creates ribonucleoprotein complexes with unspliced viral RNA. The HIV-1 Gag protein is accountable for packaging genomic RNA (gRNA) and the assembly of immature viral particles. |
| General description: | Contains amino acid residues [77-436] of HIV-1 gag antigen. |
| General description: | The human immunodeficiency virus 1 (HIV−1) gag antigen is a recombinant 39 kDa protein encoded by the GAG gene of the human immunodeficiency virus (HIV) type I expressed in and isolated from Escherichia coli. It has a β−galactosidase tag (114 kDa) on the N−terminus. HIV-1 Gag exists as monomers or lower-order oligomers in the cytosol. At the plasma membrane, it forms higher-order multimers. |
| Physical form: | Solution in 8 M urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol. |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |