HAT Media Supplement (50×) Hybri-Max™
SIGMA/H0262 - γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma
Synonym: HAT Supplement; Hypoxanthine-
Product Type: Chemical
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| form | lyophilized powder |
| impurities | endotoxin, tested |
| product line | BioXtra |
| Quality Level | 200  |
| shipped in | dry ice |
| sterility | γ-irradiated |
| storage temp. | −20°C |
| technique(s) | cell culture | hybridoma: suitable |
| Application: | HAT Media Supplement (50×) Hybri-Max™ has been used: • in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones • in the generation of mAbs (monoclonal antibodies) • in the luciferase-based bioassay (LUC) assay |
| Legal Information: | Hybri-Max is a trademark of Sigma-Aldrich Co. LLC |
| Preparation Note: | Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H360 |
| Precautionary statements | P201 - P301 + P312 + P330 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61-22 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |