Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit
SIGMA/H0412 - IgG fraction of antiserum, buffered aqueous solution
Synonym: PH3 Antibody - Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit; Ph3 Antibody; Anti-H3S10p
MDL Number: MFCD01633984
Product Type: Chemical
Immunofluorescence Whole extract of HeLa cells treated with 0.4 μg/mL nocodazole were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-phospho-Histone H3 (pSer10) antibody produced in Rabbit (Cat. No. H0412). The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Western Blotting Whole cell extract of human acute T cell leukemia Jurkat cell line treated with nocodazole were separated on SDS-PAGE and probed with Anti-phospho-Histone H3 (pSer10) antibody produced in Rabbit (Cat. No. H0412).The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 17 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Caenorhabditis elegans, bovine, human, rat, chicken, Drosophila, frog, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer10) |
| technique(s) | indirect immunofluorescence: 1:500 using human epitheloid carcinoma HeLa cell line treated with nocodazole. |
| microarray: suitable | |
| western blot: 1:2,000 using whole cell extract of human acute T cell leukemia Jurkat cell line treated with nocodazole. | |
| UniProt accession no. | P68431  |
| Application: | Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit has been used in the detection of pSer10-histone H3 by immunoblotting, immunohistochemistry and by immunofluorescence. |
| Application: | Rabbit polyclonal anti-phospho-Histone H3 (pSer10) antibody is used to tag Ser10 phosphorylated histone H3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting and immunofluorescence. It is used as a probe to determine the presence and roles of Ser10 phosphorylated histone H3 in chromosome condensation during both mitosis and meiosis. |
| Biochem/physiol Actions: | Phosphorylation of histone H3, referred to as the nucleosomal response, is localized to a small fraction of highly acetylated H3s and occurs primarily in response to mitogenic and stress stimuli. Histone H3 is phosphorylated during mitosis on at least two serine residues, Ser10 and Ser28. Phosphorylation of Ser10 is considered a crucial event for the onset of mitosis. It is tightly correlated with chromosome condensation during both mitosis and meiosis. Members of the Aurora/AIK family of protein kinases are involved in the mitotic phosphorylation of histone H3 at Ser10 residue. Expression of Aurora-A and Aurora-B is tightly coordinated with histone H3 phosphorylation at G2/M transition and both kinases efficiently phosphorylate H3 at Ser10 in vitro and in vivo. Phosphorylation of H3 at Ser10 is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. Protein kinase A (PKA), ribosomal protein S6 kinase-3 (Rsk-2) and mitogen and stress activated protein kinase-1 (Msk-1) are necessary for the histone H3 phosphorylation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the PSR-45 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with phosphoserine conjugated to KLH. Histone proteins H3, H4, H2A, H2B and H1 function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibres. The gene encoding Histone H3 is mapped to human chromosome 1q42.12. |
| General description: | Rabbit polyclonal anti-phospho-Histone H3 (pSer10) antibody recognizes histone H3 phosphorylated on Ser10 (17 kDa). Staining of pSer10-histone H3 in immunoblotting is specifically inhibited with the [pSer10] histone H3 immunizing peptide (human, amino acids 7-20). No inhibition is observed with the respective non-phosphorylated histone H3 peptide histone H3 (human, amino acids 7-20). |
| Immunogen: | synthetic, phosphorylated peptide (pSer10) histone H3 (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |