HIS-Select® HF Nickel Affinity Gel
SIGMA/H0537
Synonym: Ni-NTA HF resin
Product Type: Chemical
| capacity | 15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein) |
| form | suspension |
| matrix | Highly cross-linked 6% Beaded Agarose |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| Application: | Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions. |
| Application: | Nickel Affinity Gel for the purification of HIS TAG® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification. |
| Features and Benefits: | • High selectivity for higher purity • Unique non-charged hydrophilic linkage reduced non-specific binding • Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL • Highly cross-linked agarose matrix allows for purification under higher pressures • Binding under denaturing or non-denaturing conditions • One-step purification |
| General description: | HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr. |
| Legal Information: | FPLC is a trademark of Cytiva |
| Legal Information: | HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | 1:1 suspension in a 30% ethanol solution |
| Preparation Note: | Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided. |
| Symbol |     GHS02,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H226 - H302 + H332 - H315 - H317 - H334 - H341 - H350 - H360D - H371 - H372 - H411 |
| Precautionary statements | P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P308 + P311 |
| Hazard Codes | T,N |
| Risk Statements | 49-61-10-20/21/22-38-42/43-48/23-51/53-68-68/20/21/22 |
| Safety Statements | 53-23-36/37-45-61 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Flash Point(F) | 88.0 °F |
| Flash Point(C) | 31.1 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |