HEPES solution
SIGMA/H0887 - 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
Synonym: N-
CAS Number: 7365-45-9
MDL Number: MFCD00006158
Product Type: Chemical
| 7.5 | |
| application(s) | cell analysis life science and biopharma sample preparation |
| assay | 0.9—1.1 M |
| color | colorless |
| concentration | 1 M |
| density | 1.1 g/cm3 |
| form | liquid |
| impurities | endotoxin, tested |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1 |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA |
| pH | 7.0-7.6 |
| product line | BioReagent |
| BioXtra | |
| Quality Level | 300  |
| SMILES string | OCCN1CCN(CCS(=O)(O)=O)CC1 |
| sterility | sterile-filtered |
| storage condition | (Tightly closed. Dry) |
| dry at room temperature | |
| storage temp. | 2-8°C |
| suitability | suitable for component for culture media |
| suitable for electrophoresis | |
| technique(s) | cell culture | mammalian: suitable |
| useful pH range | 6.8-8.2 |
| Application: | HEPES has been used as a component of: • Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices • Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity • cell culture media to provide buffer capacity to maintain the pH of the cell line • buffer in decellularization of porcine nucleus pulposus |
| Disclaimer: | Concentrations of HEPES over 40mM may be toxic to some cell types. |
| Features and Benefits: | • High purity product for biochemical and biological research • Sterile filtered and cell culture tested • Ready Solution reduces the need • Useful for the pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C • Ready available solution reduce the need for preparation time |
| General description: | HEPES Buffer Solution, a sterile-filtered 1M solution of 4-(2-hydroxyethyl)piperaz Furthermore, it surpasses bicarbonate buffers (NaHCO3) in its ability to maintain physiological pH despite fluctuations in carbon dioxide concentration arising from cellular respiration, a key factor in maintaining cellular homeostasis. Beyond its exceptional performance in cell culture, HEPES may also find application in various biological and biochemical studies. It may also be valuable in techniques like immunoprecipitation, cell lysis, and live cell imaging, providing a stable and controlled environment for experimental procedures. The combination of its versatility, exceptional pH buffering capacity, and minimal interaction with other molecules positions HEPES as an indispensable tool across diverse research fields. |
| General description: | It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Preparation Note: | Prepared from HEPES free acid and cell culture grade water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 0.9—1.1 M |
| Density | 1.1 g/cm3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161704 |